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耗材專區(qū) | Empore™ E系列技術(shù)簡(jiǎn)介

更新時(shí)間：2025-01-22 點(diǎn)擊次數(shù)：409

目前蛋白質(zhì)組學(xué)耗材成本高昂。

現(xiàn)有蛋白質(zhì)組學(xué)方法需要很高的技術(shù)門檻。

需要建立簡(jiǎn)單易用和隨時(shí)可用的方法。

Empore™ E系列技術(shù)簡(jiǎn)介

E3™技術(shù)特點(diǎn)

高效：手動(dòng)操作時(shí)間<15min；
穩(wěn)定：與多種細(xì)胞裂解條件兼容（例如：尿素、SDS、RIPA、TFA等）；
經(jīng)濟(jì)：彌補(bǔ)基因組學(xué)和蛋白質(zhì)組學(xué)產(chǎn)品之間的差距；
易用：對(duì)入門級(jí)生物醫(yī)學(xué)工作者零技術(shù)障礙；
多樣：E3tips™、E3filter™和E3plate™可滿足不同的樣品體積、濃度、數(shù)量和自動(dòng)化的需求；
簡(jiǎn)便：無需液體轉(zhuǎn)移，無需考慮任何與游離磁珠相關(guān)的問題（蛋白與磁珠的比例、磁珠粘附在管壁和表面、交叉污染等）；
Empore™產(chǎn)品服務(wù)于蛋白組學(xué)領(lǐng)域20多年，有深厚的應(yīng)用基礎(chǔ)。

E3™產(chǎn)品類型

E3filter測(cè)試結(jié)果

Fig.1 Qualitative and quantitative assessment of E3technology (E3 filter) for E. coli proteome analysis. (A-C) Comparison of the number of proteins, peptides, and PSMs between the E3filter, FASP, and SP4-GB approaches. Error bars represent three replicates. (D-E) Overlapping analyses of proteins and peptides derived from the three methods. (F) Coefficient of variation of quantified proteins by the three methods. (G) Coefficient of variation of quantified peptides. (H) Pearson correlation between replicate experiments and different methods. (I) Percentages of missed cleavages. (J-K) Volcano plot of showing significantly differential proteins between the three methods. The two curves show FDR 0.05 and 0.01, respectively. (L) Heatmap of the overall quantified proteins by the three methods. FASP, filter aided sample preparation; SP4, Solvent Precipitation SP3; GB, glassbeads.

E3工作流程

E4工作流程

參考文獻(xiàn)

Yu YB, et al. Cell Reports Methods 4, 2024, 100796, June 17.
Johnston HE, et al.Anal Chem.2022, 94:10320-10328.
Wi?niewskiJR, et al. Nat Methods. 2009,6:359-62.